Liquid Chromatography-Mass Spectrometry (LC-MS/MS) is an analytical chemistry instrument that separates chemicals and aids in identifying compounds in a sample. LC-MS/MS is extremely helpful when working with isomers as such compounds can have equivalent mass and different molecular elements. Liquid Chromatography first separates the isomers allowing them to be individually analyzed during Mass Spectrometry. LC-MS/MS is able to analyze complex mixtures and provides high specificity. This technology allows multiple compounds to be measured in a single analytical run. LC-MS/MS also has the capability to switch ionization polarity modes from positive to negative at a rapid pace. This allows the chemist to test both positive and negative polarities in the same run of the sample.
LC-MS/MS is commonly used to characterize and analyze the following:
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